Apoptosis Assays In addition to our gold standard bioluminescent Caspase-Glo® Assays, we offer a wide range of kits and reagents for studying apoptosis including fluorometric and colorimetric caspase assays, TUNEL assays, apoptosis antibodies, inhibitors, and other markers for apoptosis.
“Apoptosis-like programmed cell death” refers to a cell death process that has some of the hallmarks of apoptosis such as chromatin condensation and the appearance of PS on the outer leaflet of the cell membrane but does not necessarily require caspase activity (Leist and Jäättelä, 2001).
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RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay (Cat.# JA1011 and JA1012) Includes Necrosis Detection Reagent, a cell-impermeant profluorescent DNA dye. Upon loss of membrane integrity, the dye enters the cell and binds to DNA, generating a fluorescent signal.
What if you could uncover a small but significant cellular response as your population of cells move toward apoptosis or necrosis? What if you could view the full picture of cellular changes rather than a single snapshot at one point? provided that full and clear credit is given to Promega Corporation with appropriate and specific direction
The assay provides a proluminogenic caspase-9 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 9 Reagent in an “add-mix-read” format results in cell lysis, followed by caspase cleavage of the substrate and generation of a “glow-type” luminescent signal.
The RealTime-Glo™ Apoptosis and Necrosis Assay is a simple, non-lytic assay allows real-time monitoring of apoptosis progression, without the need for multiple plates, complicated processing, or specialized detection equipment. A multimode reader capable of detecting luminescence and fluorescence is the only instrument required. The assay …
Apoptosis, or programmed cell death, is a biochemical process that occurs in multicellular organisms. This animation describes the fundamentals of the process.
Use to detect apoptosis in cultured cells and formalin-fixed, paraffin-embedded tissue sections. Plastic coverslips provided simplify sample handling. Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation.